Separation of o-Phthaldialdehyde Derivatives of Free Amino Acids from Plant Tissues by Isocratic Reverse-phase High-performance Liquid Chromatography |
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| Authors: | MARTIN, F. MAUDINAS, B. GADAL, P. |
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| Affiliation: | *Unit? de Microbiologie des Sols, INRA-CNRF Champenoux 54280, Seichamps, France
!["{dagger}"]("/math/dagger.gif") ANVAR, 93 rue de Metz, 54042 Nancy Cedex, France
!["{ddagger}"]("/math/Dagger.gif") Laboratoire de Physiologie V?g?tale M?tabolique, ERA au CNRS no. 799, Centre d'Orsay Universit? Paris-Sud, 91405 Orsay Cedex, France |
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| Abstract: | A reverse-phase high-performance liquid chromatography procedurehas been developed for rapid separation and quantitation offree amino acids as o-phthaldialdehyde derivatives. A two stepisocratic solvent system was used which enabled an accurateanalysis at nanomole level. However, two major disadvantagesto this procedure were the lack of reaction of proline and theco-elutions of threonine/glycine and tryptophan/methionine.The free amino acids in Zea mays roots were separated by usingthe method described. Amino acids, liquid chromatography, o-phthaldialdehyde derivatives, reverse-phase chromatography, Zea mays L, maize, corn |
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