家蝇卵巢在体外培育中摄取卵黄蛋白

本文报道家蝇Musca domestica卵巢在体外培养条件下，摄取异硫氰萤光素标记的家蝇卵黄蛋白的特点。用Grace's培养液标记蛋白浓度为2mg/m1，在27℃条件下培养2小时，卵巢摄取量依赖于培养液中卵黄蛋白浓度和温度，摄取高峰在羽化后48小时，正值卵母细胞发育阶段进入6-8时期。培养液中加入JHIII,能促进摄取，JHIIl的浓度和摄取量无明显相关性。乌本苷、牛血清蛋白和叠氮钠显著抑制卵巢的摄取活动。

UPTAKE OF YOLK PROTEIN BY HOUSEFLY OVARIES IN VITRO

Experiments were performed to study the incorporation of yolk protein labelled with FITC into housefly follicles in vitro. It was revealed that the rate of yolk protein incorporation depended on the concentration of yolk protein in the culture medium and temperature. The optimal condifion for yolk protein.lncorporation in this Grate's medium was 2 mg yolk protein per ml culture medium at 27℃ for twohours. The capacity of follicles in yolk protein uptake reached summit when housefly ovaries developed to the stage 6-8 about 48 hours after adult emergence.JH III, if added to the culture medium at oncentration of 5.9 to 117.3 ng/μl, could increase the amount of yolk protein incorporated into ovarian follicles. The yolk protein uptake by follicles in vitro was inhibited by ouabain, BSA or NAN₃.