Isolation and Characterization of Site-Specific DNA-methyltransferases from Bacillus coagulans K |
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| Authors: | I V Svadbina N V Zelinskaya N P Kovalevskaya L A Zheleznaya N I Matvienko |
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| Institution: | Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia. |
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| Abstract: | Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'. |
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| Keywords: | DNA methyltransferase IIS restriction endonuclease bisulfite mapping N4-methylcytosine N6-methyladenine |
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