Physical characterization of isozymes of endo-β-1,4-glucanase and β-1,4-glucosidase from Aspergillus species |
| |
| Authors: | Sunita Sharma Dhanwant K Sandhu PS Bagga |
| |
| Institution: | Microbiology Unit, School of Life Sciences, Guru Nanak Dev University, Amritsar, India. |
| |
| Abstract: | Electrophoretic data revealed the presence of various isozymes of endoglucanase and beta-glucosidase, the number of which varied from one to three in various species of the genus Aspergillus. pH 5.0 was optimum for all the isozymes whereas metal ion treatment showed complete inhibition of almost all the isozymes by Hg2+ and partial inhibition by Ca2+ and Co2+ of isozymes of both the enzymes. An alteration in the electrophoretic mobility of isozymes of beta-glucosidase was also noticed in some species with Hg2+ treatment. |
| |
| Keywords: | endo-β-1 4-Glucanase β-1 4-Glucosidase Isozyme Aspergillus Characterization |
|
|
