Identification of NRRL strain B-18602 (PR3) as Pseudomonas aeruginosa and effect of phenazine 1-carboxylic acid formation on 7,10-dihydroxy-8(E)-octadecenoic acid accumulation |
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| Authors: | C T Hou L K Nakamura D Weisleder R E Peterson M O Bagby |
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| Institution: | (1) Oil Chemical Research, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 61604 Peoria, Il, USA;(2) Microbial Properties Research, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 61604 Peoria, Il, USA;(3) Analytical Chemistry Support, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 61604 Peoria, Il, USA;(4) bioactive Constituents Research, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 61604 Peoria, Il, USA |
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| Abstract: | A new compound, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD), produced from oleic acid by a new bacterial isolate PR3, was discovered in 1991. We have now identified isolate PR3 as a strain of Pseudomonas aeruginosa by DNA reassociation studies. Strain PR3 also produced a crystalline yellowish compound the structure of which, as determined by GC/MS and NMR, is phenazine 1-carboxylic acid (PCA). In cultures of PR3, high PCA production was associated with low DOD accumulation.The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. |
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| Keywords: | Dihydroxy fatty acid phenazine 1-carboxylic acid Pseudomonas aeruginosa |
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