Regulation of the Kv2.1 Potassium Channel by MinK and MiRP1 |
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Authors: | Zoe A McCrossan Torsten K Roepke Anthony Lewis Gianina Panaghie Geoffrey W Abbott |
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Institution: | (1) Greenberg Division of Cardiology, Departments of Medicine and Pharmacology, Weill Medical College of Cornell University, Starr 463, 1300 York Avenue, 10065 New York, NY, USA;(2) Present address: Charite Campus Berlin-Buch, Experimental and Clinical Research Center, Franz Volhard Clinic and HELIOS Klinikum, Berlin-Buch, Berlin, Germany |
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Abstract: | Kv2.1 is a voltage-gated potassium (Kv) channel α-subunit expressed in mammalian heart and brain. MinK-related peptides (MiRPs),
encoded by KCNE genes, are single–transmembrane domain ancillary subunits that form complexes with Kv channel α-subunits to modify their
function. Mutations in human MinK (KCNE1) and MiRP1 (KCNE2) are associated with inherited and acquired forms of long QT syndrome
(LQTS). Here, coimmunoprecipitations from rat heart tissue suggested that both MinK and MiRP1 form native cardiac complexes
with Kv2.1. In whole-cell voltage-clamp studies of subunits expressed in CHO cells, rat MinK and MiRP1 reduced Kv2.1 current
density three- and twofold, respectively; slowed Kv2.1 activation (at +60 mV) two- and threefold, respectively; and slowed
Kv2.1 deactivation less than twofold. Human MinK slowed Kv2.1 activation 25%, while human MiRP1 slowed Kv2.1 activation and
deactivation twofold. Inherited mutations in human MinK and MiRP1, previously associated with LQTS, were also evaluated. D76N–MinK
and S74L–MinK reduced Kv2.1 current density (threefold and 40%, respectively) and slowed deactivation (60% and 80%, respectively).
Compared to wild-type human MiRP1–Kv2.1 complexes, channels formed with M54T– or I57T–MiRP1 showed greatly slowed activation
(tenfold and fivefold, respectively). The data broaden the potential roles of MinK and MiRP1 in cardiac physiology and support
the possibility that inherited mutations in either subunit could contribute to cardiac arrhythmia by multiple mechanisms.
Electronic supplementary material The online version of this article (doi: ) contains supplementary material, which is available to authorized users.
Z. A. McCrossan and T. K. Roepke have contributed equally to this work. |
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Keywords: | Potassium channel Long QT syndrome KCNE1 KCNE2 |
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