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Photo-CIDNP study of the interaction between the glucocorticoid receptor DNA-binding domain and glucocorticoid response elements |
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| Authors: | E Kellenbach T Härd R Boelens K Dahlman J Carlstedt-Duke J -Å Gustafsson G A van der Marel J H van Boom B Maler K R Yamamoto R Kaptein |
| Institution: | (1) Department of Chemistry, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands;(2) Department of Medical Nutrition and Center for Biotechnology, Karolinska Institute, Huddinge University Hospital, S-141 57 Huddinge, Sweden;(3) Gorlacus Laboratory, University of Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands;(4) Department of Biochemistry and Biophysics, University of California at San Francisco, 94143-0448 San Francisco, CA, USA |
| Abstract: | Summary Photo-CIDNP studies were performed on two protein fragments that both contain the double zinc-finger DNA-binding domain of the glucocorticoid receptor. In the absence of DNA, Tyr452 and Tyr474 are polarised in both fragments while Tyr497 is not. Addition of a palindromic glucocorticoid response element (GRE) results in the suppression of Tyr474 polarization while the polarization of Tyr452 is unaffected. The same result is observed upon adding a half GRE to the protein fragment indicating that the suppression of Tyr474 polarization is not due to protein-protein contacts but to interaction with DNA. |
| Keywords: | Glucocorticoid receptor Photo-CIDNP Glucocortoid response elements Protein-DNA interaction |
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