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Ethanol tolerance in free and sol-gel immobilised Saccharomyces cerevisiae
Authors:Martín F. Desimone  José Degrossi  Miguel D'Aquino  Luis E. Diaz
Affiliation:(1) Cátedra de Química Analítica Instrumental, Argentina;(2) Cátedra de Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 954 Piso 3, 1113 Ciudad Autónoma de Buenos Aires, Argentina
Abstract:The tolerance of sol-gel immobilised and free Saccharomyces cerevisiae to ethanol was studied. The effects of ethanol preincubation time showed that the specific death velocity decreased from 2×105 c.f.u. min–1 for free cells to 2×104 c.f.u. min–1 for immobilised cells thus indicating that immobilised yeast was far less sensitive to the ethanol damage. The specific glucose consumption of immobilised and free cells on a per cell basis was 3×10–12 g cell–1 h–1 and 9×10–12 g cell–1 h–1, respectively.
Keywords:ethanol tolerance  immobilised  Saccharomyces cerevisiae  solgel
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