Two equilibration pools of chlorophylls in the Photosystem I core antenna of Chlamydomonas reinhardtii |
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Authors: | Krzysztof Gibasiewicz V. M. Ramesh Su Lin Kevin Redding Neal W. Woodbury Andrew N. Webber |
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Affiliation: | (1) School of Life Sciences, Department of Chemistry and Biochemistry and Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, AZ 85287-4501, USA;(2) Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-4501, USA;(3) Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, AZ 85287-4501, USA;(4) Department of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznan, Poland;(5) Department of Chemistry, University of Alabama, Box 870336, Tuscaloosa, AL 35487-0336, USA |
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Abstract: | Summary Femtosecond transient absorption spectroscopy was applied for a comparative study of excitation decay in several different Photosystem I (PSI) core preparations from the green alga Chlamydomonas reinhardtii. For PSI cores with a fully interconnected network of chlorophylls, the excitation energy was equilibrated over a pool of chlorophylls absorbing at ∼683 nm, independent of excitation wavelength [Gibasiewicz et al. J Phys Chem B 105:11498–11506, 2001; J Phys Chem B 106:6322–6330, 2002]. In preparations with impaired connectivity between chlorophylls, we have found that the spectrum of chlorophylls connected to the reaction center (i.e., with ∼20 ps decay time) over which the excitation is equilibrated becomes excitation-wavelength-dependent. Excitation at 670 nm is finally equilibrated over chlorophylls absorbing at ∼675 nm, whereas excitation at 695 nm or 700 nm is equilibrated over chlorophylls absorbing at ∼683 nm. This indicates that in the vicinity of the reaction center there are two spectrally different and spatially separated pools of chlorophylls that are equally capable of effective excitation energy transfer to the reaction center. We propose that they are related to the two groups of central PSI core chlorophylls lying on the opposite sides of reaction center. |
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Keywords: | Antenna Chlorophylls Excitation energy transfer Femtosecond transient absorption Photosystem I Primary charge separation Reaction center |
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