Centrifugal column chromatography for small sample purification |
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| Authors: | W H Scouten C E Elhardt |
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| Affiliation: | Department of Chemistry, Baylor University, Waco, TX 76798. |
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| Abstract: | We evaluated several gel filtration materials for suitability in spun column chromatography a rapid desalting method. The materials were evaluated for resistance to column cracking and to radial shrinkage for sample recovery and for salt separation. A series of graphs can be derived from these evaluations which relates the gel heights to sample volumes for maximum recovery and salt separation while maintaining low sample dilution. |
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