Acetogenesis and ATP synthesis in Acetobacterium itwoodii are coupled via a transmembrane primary sodium ion gradient |
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Authors: | Reno Heise Volker Müller Gerhard Gottschalk |
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Institution: | Institut für Mikrobiologie der Georg-August-Universität, Göttingen, FRG |
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Abstract: | Abstract In cell suspensions of Acetobacterium woodii the acetyl-CoA pathway is coupled to net ATP formation. Acetate formation as well as ATP synthesis and the generation of a transmembrane sodium ion gradient are not inhibited by protonophores but by sodium ionophores. Acetogenesis from CO or formaldehyde + CO as catalyzed by inverted vesicles is coupled to sodium ion uptake. Both processes are not inhibited by protonophores but by sodium ionophores. These experiments are in accordance with the presence of a primary sodium ion pump connected to the acetyl-CoA pathway which enables the cells to synthesize net ATP by means of a Δμ Na+ in concert with a Na+-translocating ATPase. |
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