A study of histones in amoeba nuclei by indirect immunofluorescence method. |
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| Authors: | V A Sopina S B Ivanova A A Lipskaya E P Bers L G Vodop'yanova S Y Afon'kin A V Kozlov |
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| Affiliation: | 1. Laboratory of Cytology of Unicellular Organisms, Institute of Cytology of the Academy of Sciences of the USSR, Leningrad 190121, USSR;2. Laboratory of Algae Biochemistry, Biological Research Institute of Leningrad State University, Leningrad 199164, USSR |
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| Abstract: | Nuclear proteins of four species of free-living Amoebidae (Amoeba proteus, A. discoides, Chaos carolinensis and Polychaos dubia) have been studied by indirect immunofluorescence technique using specific antisera to H1, H2A, H2B, H3 and H4 histone fractions from the calf thymus. It has been shown that the nuclei of the species examined have all these five histone fractions. However, the degree of similarity between homologous fractions from amoebae and the calf thymus varies and can be expressed in terms of immunological distance. Immunological differences between amoebic and calf thymus histones are the most pronounced in H1, being least in H3 and H4. Judged by its immunochemical characteristics, the histone fraction H2A from P. dubia is closer to the corresponding fraction from the calf thymus than is H2A from the other three amoeba species. |
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