Immunological properties of Fc receptor on lymphocytes. 6. Characterization of suppressive B-cell factor (SBF) released from Fc receptor-bearing B cells. |
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Authors: | M Miyama J Yamada T Masuda |
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Institution: | 1. Institute for Immunology, Faculty of Medicine, Kyoto University, Yoshida, Sakyo, Kyoto 606, Japan;2. Institute for Experimental Animals, Faculty of Medicine, Kyoto University, Yoshida, Sakyo, Kyoto 606, Japan |
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Abstract: | EA, i.e., antigen-antibody complexes are able to induce an antigen-nonspecific suppressive factor(s) from FcR+ B cells by binding on FcR. This factor, termed “suppressive B-cell factor (SBF)” was only effective on H-2 compatible, but not on H-2 incompatible spleen cells in an adoptive cell transfer system. Furthermore, SBF, prepared from B10.A (H-2a) splenic FcR+ B cells, suppressed the adoptive primary response of B10.D2 mice (H-2d), in addition to A/J mice (H-2a) against DNP-DE, by the pretreatment of cells with SBF in vitro. Absorption with affinity columns demonstrated that active components) of SBF from C3H/He mice (H-2k) was eliminated by both B6 anti-CBA (H-2b anti-H-2k) and B10.D2 anti-B10.BR (H-2d anti-H-2k), but not B10 anti-B10.A (H-2b anti-H-2a). In contrast, the suppressive activity of SBF was eliminated neither by anti-mouse Ig nor by a heat-aggregated human γ-globulin column. These results indicate that SBF contains a product coded by the right-hand side of H-2 gene complex, but does not contain Ig determinants nor FcR. Thus, it is conceivable that a compatibility of the right-hand side of H-2 gene complex is required for inducing effective suppression of spleen cells by SBF. SBF was considered to be a trypsin-resistant and heat-labile substance with a molecular weight of 30,000–63,000. The target cells for SBF were FcR? B precursors, but not helper T cells. |
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Keywords: | DNP dinitrophenyl Asc KLH keyhole limpet hemocyanin nonbearing and bearing Fc receptor PFC plaque forming cells Ig immunoglobulin SRBC sheep red blood cell EA sheep erythrocytes sensitized with 7 S rabbit anti-SRBC agg-HGG heat-aggregated human γ-globulin MEM Eagle's minimum essential medium SBF suppressive B-cell factor |
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