Inhibition of translation in L-cell lysates by free polyadenylic acid: differences in sensitivity among different mRNAs and possible involvement of an initiation factor |
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| Authors: | G Lemay S Millward |
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| Institution: | 1. Division of Biliary Tract Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China;2. Center for Translational Medicine, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China;1. Federal Institute for Risk Assessment, Berlin, Germany;2. Friedrich-Loeffler-Institut, Institute for Novel and Emerging Infectious Diseases, Greifswald-Insel Riems, Germany;3. University of Bern, Institute of Ecology and Evolution, Bern, Switzerland;4. Swiss Institute of Bioinformatics, Genopode, Lausanne, Switzerland;5. Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine (Vetmeduni Vienna), Vienna, Austria;1. Department of Immunology, College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China;2. Center for Tissue Engineering and Stem Cell Research , Guizhou Medical University, Guiyang 550004, China;3. Key Laboratory of Adult Stem Cell Translational Research (Chinese Academy of Medical Sciences), Guiyang 550004, China;4. Department of Pharmacology, Guizhou Medical University, Guiyang 550025, China;5. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China;1. Departments of Neurology and Medicine, 1225 Morris Park Avenue, Room 316 Bronx, New York, 10461, USA;2. Albert Einstein College of Medicine, USA |
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| Abstract: | Free polyadenylic acid specifically inhibits in vitro translation of naturally polyadenylated mRNAs in L-cell lysates. The polynucleotide affects the initiation of protein synthesis but has no apparent effect on elongation of polypeptide chains. Reovirus mRNA, naturally devoid of a poly(A) tail, is much less sensitive to this inhibition than are naturally polyadenylated mRNAs. Reovirus mRNA that was polyadenylated in vitro is not more sensitive than normal reovirus mRNA. The degree of inhibition of translation varies for the different reovirus mRNA species. The addition of proteins contained in a high salt wash of ribosomes can mitigate the inhibition of translation of naturally polyadenylated mRNAs by free polyadenylic acid. Altogether these results suggest that the inhibition by polyadenylic acid may be mediated by its interaction with a cellular (initiation) factor. The various sensitivities exhibited by different mRNAs may indicate differences in requirement for this factor. |
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