Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network |
| |
Authors: | Yeaman Charles Ayala M Inmaculada Wright Jessica R Bard Frederic Bossard Carine Ang Agnes Maeda Yusuke Seufferlein Thomas Mellman Ira Nelson W James Malhotra Vivek |
| |
Institution: | Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242-1109, USA. |
| |
Abstract: | Protein kinase D (PKD) binds to diacylglycerol (DAG) in the trans-Golgi network (TGN) and is activated by trimeric G-protein subunits beta gamma. This complex then regulates the formation of transport carriers in the TGN that traffic to the plasma membrane in non-polarized cells. Here we report specificity of different PKD isoforms in regulating protein trafficking from the TGN. Kinase-inactive forms of PKD1, PKD2 and PKD3 localize to the TGN in polarized and non-polarized cells. PKD activity is required only for the transport of proteins containing basolateral sorting information, and seems to be cargo specific. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|