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Cloning and sequence analysis of the Candida utilis HIS3 gene
Authors:Basabe Liliana  Domínguez Iveldris  Chávez Francisco P
Institution:Center for Genetic Engineering and Biotechnology, Agriculture Division, 31 Ave /158 & 190, PO Box 6162, Havana 10600, Cuba. liliana.basabe@cigb.edu.cu
Abstract:A DNA fragment, carrying the Candida utilis HIS3 gene, has been isolated from a genomic DNA library by complementation of the E. coli hisB mutant. Its nucleotide sequence was determined and it predicts a single open reading frame of 675 bp (224 aa). The deduced amino acid sequence is highly homologous to other yeast and fungi HIS3 genes.
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