高速逆流色谱结合半制备液相色谱制备甘青青兰中3种活性成分

采用高速逆流色谱（HSCCC）结合液相色谱法制备甘青青兰（Dracocephalum tanguticum Maxim.）中绿原酸、胡麻甙-6″-乙酯、迷迭香酸，建立快速分离制备甘青青兰中活性成分的方法。采用半制备型高效液相色谱（SP-HPLC）富集甘青青兰乙酸乙酯萃取物中绿原酸、胡麻甙-6″-乙酯、迷迭香酸，再用制备液相（Pre-HPLC）和HSCCC对富集物进行分离纯化，获得54 mg化合物Ⅰ、130 mg化合物Ⅱ和200 mg化合物Ⅲ，纯度分别为96.9%、97.9%和95.1%，经核磁共振碳谱（¹³CNMR）和氢谱（¹HNMR）分别鉴定为绿原酸、胡麻甙-6″-乙酯和迷迭香酸。本实验方法适用于甘青青兰乙酸乙酯萃取物中绿原酸、胡麻甙-6″-乙酯和迷迭香酸的分离制备，并避免了传统分离方法操作繁多、试剂消耗量大、不可回收等弊端，为分离甘青青兰活性成分、制备对照品等研究提供了参考依据。

Separation and Purification of Three Active Components from Dracocephalum tanguticum Maxim.by High-Speed Counter-current Chromatography Combined with Semi-Preparative High Performance Liquid Chromatography

An efficient method was established for the separation and preparation of chlorogenic acid,pedaliin-6″-acetate and rosmarinic acid from Dracocephalum tanguticum Maxim..The chlorogenic acid,pedaliin-6″-acetate and rosmarinic acid was enriched by semi-preparative high performance liquid chromatography(SP-HPLC)from ethyl acetate extract,and then separated and purification by high-speed counter-current chromatography(HSCCC)and preparative high performance liquid chromatography(Pre-HPLC).A total of 54 mg of chlorogenic acid,150 mg of pedaliin-6″-acetate and 200 mg of rosmarnic acid were obtained from ethyl acetate extract with purities of 96.9%,97.9%and 95.1%by HPLC analysis,respectively.Their structures were identified by nuclear magnetic resonance spectrum.The result show that this method is suitable for the isolation and purification of chlorogenic acid,pedaliin-6″-acetate and rosmarnic acid from D.tanguticum.It also avoids the disadvantages of traditional separation methods such as many operations,large reagent consumption and non-recyclability,provides a reference basis for the study of separating of active components and preparation reference substance from D.tanguticum.