InSaccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions |
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| Authors: | Paola Cavadini Laura Popolo Marina Vai Rosa Varona Luisa Garofano Marco Vanoni |
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| Affiliation: | (1) Dipartimento di Fisiologia e Biochimica Generali, Sezione di Biochimica Comparata, Università degli Studi di Milano, Via Celoria, 26 20133 Milano, Italy;(2) Pharmacia Antibioticos, S.A. R&D Dept., Antonio Lopez, 28026 Madrid, Spain;(3) Pharmacia BioScience Center R&D Corporate, via Giovanni XXIII, Nerviano, Italy |
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| Abstract: | Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose  galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release. |
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