Characterization of H2O2-induced acute apoptosis in cultured neural stem/progenitor cells |
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Authors: | Lin Hsingchi J Wang Xiantao Shaffer Kara M Sasaki Carl Y Ma Wu |
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Affiliation: | Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA. jlin@cbmse.nrl.navy.mil |
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Abstract: | In the present study, we characterized hydrogen peroxide (H2O2)-induced cell apoptosis and related cell signaling pathways in cultured embryonic neural stem/progenitor cells (NS/PCs). Our data indicated that H2O2 induced acute cell apoptosis in NS/PC in concentration- and time-dependent manners and selectively, it transiently increased PI3K-Akt and Mek-Erk1/2 in a dose-dependent manner. Inhibition of PI3K-Akt with wortmannin, a PI3-K inhibitor, was found to significantly increase H2O2-induced acute apoptosis and dramatically decrease basal pGSK3β levels. The level of pGSK3β remained unchanged with H2O2 exposure. We conclude that the transient activation of PI3K-Akt signaling delays the H2O2-induced acute apoptosis in cultured NS/PCs in part through maintaining the basal pGSK3β level and activating other downstream effectors. |
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Keywords: | Oxidative stress Apoptosis Akt Survival Neural stem cell Erk1/2 |
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