Sox10-rtTA mouse line for tetracycline-inducible expression of transgenes in neural crest cells and oligodendrocytes |
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Authors: | Ludwig Andreas Schlierf Beate Schardt Anke Nave Klaus-Armin Wegner Michael |
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Affiliation: | Institut für Biochemie, Universit?t Erlangen-Nürnberg, Fahrstrasse 17, 91054 Erlangen, Germany. |
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Abstract: | Using gene targeting, we inserted a high-affinity variant of the reverse tetracycline controlled transactivator (rtTA) into the genomic Sox10 locus. This rtTA transgene faithfully recapitulated Sox10 expression in the emerging neural crest, several of its derivatives, and in oligodendrocytes. It was furthermore able to induce expression of a tetracycline inducible transgenic reporter gene in a doxycycline-dependent manner. Induction was fast, with substantial reporter gene expression visible 6 h after the onset of doxycycline treatment. Shut-off, in contrast, exhibited delayed kinetics, which probably correlated with doxycycline clearance rates. This mouse provides a useful tool for generating tetracycline-controlled gene expression in neural crest and oligodendrocytes. |
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Keywords: | Sry high‐mobility group Tet‐on Schwann cell melanocyte enteric nervous system |
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