An improved system of subjecting plants to water stress |
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Authors: | P N Singh R Prasad M Salim A Sharga |
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Institution: | (1) Plant Physiology Laboratory, Department of Botany, University of Allahabad, 211 002 Allahabad, India |
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Abstract: | An improved system of plant cultivation at stable and specific levels of polyethyleneglycol (PEG, mol. mass 1400–1600) — induced
water stress has been described. To set up this system a perforated tubular glass vessel containing soil to support seedling
growth was wrapped externally first with a layer of macroporous silica gel-G and then with three layers of a dialysis membrane
of a lower exclusion limit (2000 mol. mass). Effects of 8 days of PEG — induced stress have been studied on uptake and translocation
of N and P and growth of barley (Hordeum vulgare L. cv. KN 16) seedlings. Some of the noteworthy improvements of the system were exelusion of PEG from the plant consequent
upon use of silica gel-membrane combination, shorter time (2 days) for the soil-plant-air continuum to attain steady state,
and stability of the plant water potential over a period of a few days. |
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