Cell-Type Dependence of Stability Modulation of Lectin-initiated Contacts by Impairment of Multivalent Carbohydrate Binding and Intracellular Signaling |
| |
Authors: | A V Timoshenko I V Gorudko S André H-J Gabius |
| |
Institution: | (1) Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, 220072 Minsk, Belarus E-mail;(2) Department of Biophysics, Belarusian State University, Pr. F. Skaryny 4, 220050 Minsk, Belarus;(3) Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, 80539 Munich, Germany |
| |
Abstract: | Initial ligand selection and the intermolecular spatial arrangement ofglycan-lectin complexes are assumed to be essential to induce formationof stable cell aggregates by a lectin. To distinguish effects of thesetwo processes, the tetrameric mistletoe lectin and its isolated B-chainwere used. A reduced impact of multivalency for Ehrlich ascites tumorcells in contrast to rat thymocytes was revealed. Signaling is thusinitiated in a cell-type-dependent manner. Using selective metabolicinhibitors to reduce signal transfer for aggregate stability, decreasein cellular SH-group level was shown to be a common effect accompanyingsuppression of lectin-dependent aggregate stability. The resultsunderscore an intrinsic variability in the relative importance oflectin-dependent glycan aggregation on the cell surface for triggeringpost-binding lectin effects. |
| |
Keywords: | cell adhesion Ehrlich ascites carcinoma lectin mistletoe signaling inhibitor thymocytes |
本文献已被 PubMed SpringerLink 等数据库收录! |
|