Density of NMDA-Coupled and Uncoupled 1-[1-(2-[3H]Thienyl)cyclohexyl]piperidine Recognition Sites in the Brain and Spinal Cord: Differential Effects of NMDA Agonists and Antagonists |
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Authors: | Katalin J Kovács Alice A Larson |
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Institution: | Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A. |
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Abstract: | Abstract: Binding of 1-1-(2-3H]thienyl)cyclohexyl]piperidine (3H]TCP) to mouse brain and spinal cord membranes was studied using compounds selective for the NMDA-coupled 1-(1-phenylcyclohexyl)piperidine (PCP) and/or σ recognition sites. In both tissues, 3H]TCP labeled two populations of binding sites. Density of the low-affinity sites was approximately the same in both tissues, but the population of the high-affinity 3H]TCP sites was three times bigger in the brain than in the spinal cord. Self- and cross-displacement studies showed that the high-affinity 3H]TCP binding sites could be identical with NMDA receptor-coupled PCP sites, whereas the low-affinity 3H]TCP sites may be associated with σ binding sites in both tissues. The NMDA-coupled PCP sites labeled in the presence of 6.25 n M 3H]TCP constituted a much higher percentage of the total binding in the brain (75%) than in the spinal cord (44%). Consistent with this, reintroduction of glycine and glutamate significantly increased, but DA antagonists significantly inhibited 3H]TCP binding in the brain but not in the spinal cord. Together, these data suggest that a large component of 3H]TCP-labeled binding sites in the spinal cord may be associated with σ but not the NMDA receptor-coupled PCP sites. |
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Keywords: | 1-[1-(2-[3H]Thienyl)cyclohexyl]piperidine binding NMDA σ Sites Brain Spinal cord MK-801 |
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