树莓粗多糖对UVB诱导HaCaT细胞光损伤的防护作用

多糖是树莓功能性成分之一,具有抗炎、抗氧化、抗疲劳、降血糖、免疫调节等多种药理作用。但树莓多糖对紫外线造成的皮肤细胞光损伤是否具有防护作用尚未见报道。本研究旨在探究树莓粗多糖(raspberry crude polysaccharide,RCP)对中波紫外线(ultraviolet B,UVB)诱导的人永生化角质形成细胞(human immortalized keratinocytes,HaCaT)光损伤的防护作用。通过建立HaCaT细胞的UVB光损伤模型,利用CCK-8法测定细胞活力,酶联免疫吸附法(enzyme linked-immuno-sorbent assay,ELISA)和微板法测定HaCaT细胞中炎症因子、基质金属蛋白酶和抗氧化因子的含量,评估RCP的抗UVB活性。采用体外自由基清除实验检测RCP对DPPH自由基(DPPH·)和ABTS自由基(ABTS·⁺)的清除能力。结果表明,RCP能明显提高被UVB损伤的HaCaT细胞活力,且随浓度升高作用增强(P < 0.01或P < 0.05)。与未加入RCP处理的HaCaT细胞比较,人基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)、人白介素-1β(interleukin-1β,IL-1β)、人白介素-6(interleukin-6,IL-6)、人肿瘤坏死因子-α(tumor necrosis factor α,TNF-α)的含量明显降低(P < 0.01或P < 0.05),超氧化物歧化酶(superoxide dismutase,SOD)、还原型谷胱甘肽(glutathione,GSH)以及人硫氧还蛋白(thioredoxin,TRX)的含量显著升高(P < 0.05)。此外,RCP对DPPH·和ABTS·⁺的最大清除率分别为91%和94%。以上结果表明,RCP能够通过降低炎症水平和缓解氧化应激来预防UVB对HaCaT细胞造成的光损伤,为天然抗UVB物质的研发提供新思路,也为树莓资源的高值化利用和精深开发提供参考。

Protective Effects of Raspberry Crude Polysaccharides on UVB-Induced Photodamage in HaCaT Cells

Polysaccharide is one of the functional components of the raspberry, which has various pharmacological effects such as anti-inflammatory, antioxidant, anti-fatigue, hypoglycemic and immunomodulatory. However, whether raspberry polysaccharides have protective effects on UV-induced photodamage to skin cells has not been reported. This study aims to investigate the protective effect of Raspberry Crude Polysaccharide on Ultraviolet B (UVB)-induced photodamage of human immortalized keratinocytes (HaCaT). The photodamage model of HaCaT cells was established by UVB irradiation. To evaluate the anti-UVB activity of RCP, the cell viability was determined by the CCK-8 method, and the Enzyme-linked immunosorbent assays (ELISA) and microplate method were used to measure the contents of matrix metalloproteinase, inflammatory and antioxidant factors in the photodamaged HaCaT cells. The antioxidant activity of RCP was detected by radical scavenging assays against DPPH radical (DPPH·) and ABTS radical (ABTS·⁺) in vitro. The results showed that RCP could significantly increase the viability of UVB-injured HaCaT cells, and the effect was enhanced with the increase of its concentration (P < 0.01 or P < 0.05). Compared with HaCaT cells without RCP treatment, human matrix metalloproteinase-3 (MMP-3), human interleukin-1β (IL-1β), human interleukin-6 (IL-6), human tumor necrosis factor-α (TNF-α) were obviously decreased (P < 0.01 or P < 0.05), the superoxide dismutase (SOD), reduced glutathione (GSH) and human thioredoxin (TRX) content was significantly increased (P < 0.05). Furthermore, the maximal clearance of DPPH· and ABTS·⁺ by RCP was 91% and 94%, respectively. In conclusion, our experimental results show that RCP can prevent the photodamage of HaCaT cells caused by UVB by reducing the level of inflammation and alleviating oxidative stress, providing new ideas for the research and development of natural anti-UVB substances, and also for the highly valued utilization and development of raspberry resources.