三元基序家族蛋白15敲低诱导肝癌细胞周期阻滞

三元基序家族蛋白15 (tripartite motif-containing protein 15,TRIM15)是TRIM家族成员,该家族是一类具有E3泛素连接酶活性的蛋白质.TRIM15在肿瘤中的功能鲜有报导.本研究意在阐释TRIM15在肝细胞癌(hepatocellular carcinoma,HCC)中的作用...

TRIM15 Knockdown Induces Cell Cycle Arrest in Hepatocellular Carcinoma Cells

Tripartite motif-containing protein 15 (TRIM15) is a member of the TRIM family, which is a class of proteins with E3 ubiquitin ligase activity. The function of TRIM15 in tumors is rarely reported. This study is intended to explain the role of TRIM15 in hepatocellular carcinoma. Nuclear and cytoplasmic fractionation and immunofluorescence assays confirmed that TRIM15 was located in the nucleus and cytoplasm. We designed hairpin RNA (shRNA) to knockdown TRIM15 in hepatocarcinoma cell lines. After knocking down TRIM15, cell growth curve and clone formation assays showed that cell proliferation was significantly inhibited (P<0.05). Cell cycle analysis by flow cytometry showed that knockdown of TRIM15 blocked cell cycle in the G₀/G₁ phase (P<0.05). After knocking down TRIM15, the expression of Cyclin A, CDK2, Rb, P-Rb, and E2F1 were down-regulated, while the expression of CKI p16, p21, and p27 were significantly up-regulated. These findings suggest that knockdown TRIM15 induces cell cycle arrest of liver cancer cells, which indicates that TRIM15 can be used as a feasible target for the treatment of hepatocellular carcinoma.