Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees |
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| Authors: | Gang Xin Dean Glawe Sharon L Doty |
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| Institution: | aDepartment of Civil and Environmental Engineering, University of Washington, Seattle 98195, USA;bDepartment of Plant Pathology, Washington State University, University of Washington, Seattle 98195, USA;cCollege of Forest Resources, University of Washington, Seattle 98195, USA |
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| Abstract: | Three endophytic yeast, one isolated from stems of wild cottonwood (Populus trichocarpa), two from stems of hybrid poplar (P. trichocarpa × Populus deltoides), were characterized by analyzing three ribosomal genes, the small subunit (18S), internal transcribed spacer (ITS), and D1/D2 region of the large subunit (26S). Phenotypic characteristics of the yeast isolates were also obtained using a commercial yeast identification kit and used for assisting the species identification. The isolate from wild cottonwood was identified to be closest to species Rhodotorula graminis. The two isolates from hybrid poplar were identified to be species Rhodotorula mucilaginosa. In addition, the three yeast isolates were observed to be able to produce indole-3-acetic acid (IAA), a phytohormone which can promote plant growth, when incubated with l-tryptophan. To our knowledge, the yeast strains presented in this study were the first endophytic yeast strains isolated from species of Populus. |
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| Keywords: | Endophyte IAA ITS Poplar Rhodotorula |
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