Endogenous formation of prostanoids and the levels of long-chain fatty acyl CoA derivatives in suspensive and adhesive cells from in vitro embryoid bodies of teratoma OTT 6050

Attachment of the cell surface to a substratum may play a critical role in initiating some cellular developmental commitments and in sustaining differentiation of cells that have already been specialized. Embryoid bodies of teratoma OTT6050 were divided, on day 10 of initial culture, into myogenic adhesive cells which were already (at day 6) characterized by endogenous prostaglandin (PG)I₂ formation and little-specialized suspensive cells which formed only thromboxane (TX)B₂ in the same culture system. Since at day 10 both cell types reached a stationary phase in which the nature of each cell was mature enough for the analyses with mass fragmentographic technique and gas chromatography- mass spectrometry (GCMS), the total levels of predominant long-chain fatty acyl CoA (acyl CoA) derivatives could be measured comparatively as methyl esters after methanolysis. It was found as a result of major differentiation that adhesive cells had a rather low ratio of arachidonyl CoA to stearyl CoA, although adhesive cells accumulated a larger total amount of acyl CoA derivatives than that accumulated in suspensive cells.