Application of immobilized aminoacylase from Micrococcus agilis for isolation of D-phenylglycine from its racemic mixture

The procedure for isolation of D -phenylglycine from its racemic mixture by enzymatic hydrolysis of L -enantiomer of N-acetyl-D ,L -phenylglycine is described. For this hydrolysis. aminoacylase from Micrococcus agilis immobilized by sorption of DEAE-cellulose was applied. As is also shown, the course of enzymatic reaction can be directly controlled by spectrophotometric method.