| Abstract: | Methods of determining the numerical density of cell organelles described in literature were critically reviewed in a morphometrical and stereological study of muscle cell mitochondria (heart muscle cells, diaphragm cells, sceletal muscle cells). A review of the method described by WEIBEL and GOMEZ showed that the numerical density of the mitochondria depends to a great extent on their shape and not so much on their size distribution. For this reason serial sections should be used to determine the shape factor in biological objects of unknown geometric shape. Generally, the numerical densities of mitochondria determined by using the method proposed by DEHOFF and RHINES were higher than those obtained with the method described by WEIBEL and GOMEZ. This is attributed to certain corrections used in the former method. Elaborate computations are generally involved and the geometric shapes of the object examined must be known in order to determine the numerical density of cell organelles or of other biological structures. The numerous sources of error involved in these methods give this parameter the character of an objective estimate. For this reason it is recommended that the value obtained should be checked by determining a two-dimensional parameter. Our examinations of heart muscle mitochondria showed good agreement between the two parameters. |
