Peptide synthesis enzymatically catalyzed in a biphasic system: Water–water–immiscible organic solvent

Synthesis of a peptide bond is suggested to be enzymatically catalysed in a biphasic system “water–water-immiscible organic solvent”. The pH dependence of the apparent equilibrium constant is studied for synthesis of N-acetyl-L -tryptophanyl-L -leucine amide from N-Acet-Trp andL -Leu-NH₂. The reaction was performed in the biphasic system ethyl acetate plus water from 2 to 2% (v/v)] in the presence of α-chymotrypsin. The suggested approach is preparative value: with the stoichiometric ratio of the reagents, N-Acet-L -Trp] = L -Leu-NH₂] = 2 × 10^?3M, the yield is practically 100% (in water, with other conditions being the same, the yield is not over 01.%).