The isomerization of D-glucose into D-fructose catalyzed by whole-cell immobilized glucose isomerase. The dependence of the intrinsic rate of reaction on substrate concentration,pH, and temperature

The relation between the intrinsic rate of the glucose-fructose isomerization catalyzed by whole-cell immobilized glucose isomerase and the substrate concentration can be described with the kinetic model The numerical values of k₁, k_?1, k₂, and k_?2 have been determined from low-conversion experiments starting from pure glucose or fructose solutions, and are presented as a function of pH and temperature. The difference between the overall chemical reaction rate determined in high-conversion experiments and that calculated from the individual k₁, k_?1, k₂, and k_?2 values is less than 10%.