矮丛蓝莓叶片的愈伤组织诱导及植株再生

以矮丛蓝莓'Blomidon'品种的试管苗叶片为外植体,研究不同培养基、激素、转接时间以及转接方式对愈伤组织诱导及植株再生的影响.结果表明:WPM1/2为适宜的培养基;添加0.5 mg·L~(-1) CPPU+1.0 mg·L~(-1) ZT+0.5 mg·L~(-1) TDZ和0.5 mg·L~(-1) CPPU+0.5 mg·L~(-1) ZT+1.0 mg·L~(-1) TDZ诱导愈伤率较高达100%;ZT、CPPU与生长素IAA、IBA的配比诱导愈伤率效果不佳;添加ZT诱导的愈伤组织适合20 d后转接,CPPU诱导的愈伤组织适合50 d后转接;转接方式均以固体培养为宜.矮丛蓝莓'Blomidon'最适宜培养基为WPM1/2+0.5 mg·L~(-1) CPPU+1.0 mg·L~(-1) ZT+0.5 mg·L~(-1) TDZ,其诱导愈伤组织50 d后转接平均分化芽数最多达10.26个.

Callus Induction and Plant Regeneration of Vaccinium angustifolium Leaves

Plantlet leaves in vitro of Vaccinium angustifolium 'Blomidon' were used as the explants,effects of the different media,hormones,switching time and switching methods on callus induction and plant regeneration were studied.The results showed that:WPM1/2 was a suitable culture medium;media with 0.5 mg·L~(-1) CPPU+1.0 mg·L~(-1) ZT+0.5 mg·L~(-1) TDZ和0.5 mg·L~(-1) CPPU+0.5 mg·L~(-1) ZT+1.0 mg·L~(-1) TDZ had a 100% rate for callus induction callus;The combination of ZT,CPPU with the auxin IAA,IBA could not effectively induced callus;Add the ZT and CPPU induced callus were switched,the appropriate time for callus culture was 20 d and 50 d,respectively.Compared with the solution medium,the solid culture method was better.The above results indicated that the most suitable medium was WPM1/2+0.5 mg·L~(-1) CPPU+1.0 mg·L~(-1) ZT+0.5 mg·L~(-1) TDZ for Vaccinium angustifolium 'Blomidon',which the average number of adventitious buds up to 10.26 when the callus was switched after 50 d.