Metabolism of 25-hydroxyvitamin D3 in kidney homogenates of chicks supplemented with vitamin D3. |
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Authors: | Y Takasaki N Horiuchi T Suda |
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Institution: | Department of Biochemistry, Showa University Dental School, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo 142, Japan |
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Abstract: | Kidney homogenates from chicks fed a vitamin D-deficient diet for 10 days and supplemented with 6.5 nmol of vitamin D3 48 hr prior to sacrifice metabolized -25-hydroxyvitamin D3 (25-OH-D3) to 24,25-dihydroxyvitamin D3 24,25-(OH)2-D3] and 3 other metabolites (peaks A, C and E). When the homogenates were incubated with purified 3H]-24,25-(OH)2-D3, 3 similar metabolites (peaks A′, C′ and E′) were produced. On high pressure liquid chromatography, peaks A, C and E migrated to exactly the same respective positions as peaks A′, C′ and E′. Kidney homogenates from D-deficient chicks failed to produce these metabolites from 3H]-25-OH-D3 or 3H]-24,25-(OH)2-D3. These results strongly suggest that the new metabolites reported here are synthesized via 24,25-(OH)2-D3 in the kidney of chicks supplemented with vitamin D3. |
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Keywords: | To whom all correspondence should be addressed |
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