Tumor reactivity of immune T cells in short-term culture |
| |
Authors: | J C Krauss Jeannine M Stein Suyu Shu |
| |
Institution: | (1) The Center for Surgery Research/FF-50, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195, USA Fax: (216) 445 3805 e-mail kraussj@cesmtp.ccf.org, US |
| |
Abstract: | The adoptive transfer of immune T cells is capable of mediating the regression of established neoplasms in a variety of animal
tumor models. The antitumor activity is invariably proportional to the number of cells transferred, thus methods to expand
immune cell number while maintaining therapeutic efficacy have been extensively investigated. Here we demonstrate that a short-term
culture of immune T cells can amplify the T cell number and enhance the therapeutic reactivity against established pulmonary
tumor, while maintaining immunological specificity. In contrast, the therapeutic reactivity of immune T cells against established
subcutaneous tumor is diminished by short-term culture. While cultured immune T cells are not cytotoxic in a 4-h Cr-release
assay, they do specifically secrete interferon γ upon stimulation with tumor cells. T cells cultured after a single exposure
to tumor are even more active against pulmonary tumor than T cells cultured from mice immunized repeatedly. This culture system
can rapidly induce T cell proliferation and differentiation into mature effector cells, and the resulting cells demonstrate
an enhanced ability to treat visceral metastases, but a decreased ability to treat subcutaneous tumor. Thus T cells cultured
after a single exposure to tumor represent an ideal population of cells for use in human adoptive immunotherapy trials.
Received: 18 July 1996 / Accepted: 27 September 1996 |
| |
Keywords: | Murine Immunotherapy CD3 IL-2 Interferon γ |
本文献已被 SpringerLink 等数据库收录! |
|