Evidence for an increase in X-ray-induced translocation frequency in oocytes of caffeine-treated Drosophila females |
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| Authors: | C Osgood S Zimmering |
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| Affiliation: | Biomedical Sciences Division, Lawrence Livermore Laboratory, University of California, Livermore, CA 94550, U.S.A. |
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| Abstract: | 0-8 h old Drosophila females carrying a reversed metacentric X chromosome and a suitably marked Y chromosome were treated or not with 0.2% caffeine and irradiated with 2000 R X-rays. In contrast with the reduction found in translocation frequency following 2000 R irradiation of the male mated with 0.2% caffeine-treated females, the frequency of interchanges in oocytes was significantly higher with caffeine as compared with controls. |
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| Keywords: | α-MEM alpha minimum essential Eagle medium AA 8-azaadenine AG 8-azaguanine APRT adenine phosphoribosyltransferase, EC 2.4.2.7 BP BUdR 5-bromodeoxyuridine CHO Chinese hamster ovary DFCS dialyzed fetal-calf serum DMN dimethylnitrosamine EMS ethyl methanesulfonate FCS fetal-calf serum HAT hypoxanthine-amethopterin-thymidine HGPRT hypoxanthine—guanine phosphoribosyltransferase, EC 2.4.2.8 FUdR 5-fluorodeoxyuridine density of cells yielding 50% mutant survival in drug-selection experiments MNNG NQO 4-nitroquinoline 1-oxide OUA ouabain octa-hydrate hydrate PBS phosphate-buffered saline S.D. one standard deviation SEM one standard error of the mean TG 6-thioguanine TFT trifluorothymidine TK thymidine kinase, EC 2.7.1.21 WT Correspondence to: June H. Carver, Battelle-Columbus Laboratories, Biomedical Sciences Division, 505 King Avenue, Columbus, Ohio 43201 (U.S.A.) |
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