Genetic diversity in the Japanese rosy bitterling, Rhodeus ocellatus kurumeus (Cyprinidae) |
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Authors: | Kouichi Kawamura Yoshikazu Nagata Hiroyuki Ohtaka Yoshihiko Kanoh Jyun-ichi Kitamura |
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Institution: | (1) Division of Genetics, National Research Institute of Aquaculture, Nakatsuhamaura, Nansei, Watarai, Mie 516-0193, Japan (e-mail: barabote@fra.affrc.go.jp), JP;(2) Department of Biology, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan, JP;(3) Yashima-nishi Elementary School, Yashima-nishi, Takamatsu, Kagawa 761-0013, Japan, JP;(4) Section of Biology, Seifu High School, 12-16 Ishigatsuji, Tennohji, Osaka 543-0031, Japan, JP;(5) Laboratory of Animal Ecology, Faculty of Science, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto 606-8502, Japan, JP |
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Abstract: | Comparison of meristic characters (pored lateral line scales, vertebrae, and fin rays), and PCR-RFLP analysis in the D-loop
and ND1 regions of mitochondrial DNA were performed to estimate the genetic diversity in local populations of the Japanese
rosy bitterling, Rhodeus ocellatus kurumeus. In terms of meristic characters, the Fukuoka population was the largest in both range and variance of the number of pored
lateral line scales and vertebrae (abdominal and caudal), and Osaka was the second, whereas the Kagawa population showed the
smallest range and variance in these characters. In PCR-RFLP analysis, 11 haplotypes (3 in Fukuoka, 2 in Okayama, 2 in Kagawa,
and 4 in Osaka) were observed, and nucleotide sequence divergence (NSD) was approximately two times larger in ND1 (mean, 0.61%)
than in D-loop (mean, 0.31%). In the neighbor-joining (NJ) tree, based upon the NSD value in ND1, haplotypes were arranged
into four clades, which corresponded to the locality of each haplotype. The Fukuoka population was conspicuously apart from
the other populations (mean, 0.90% in NSD), but the remaining three showed a similar genetic distance with each other (mean,
0.48%–0.52% in NSD). In haplotype diversity of mtDNA, half the stations in Osaka and all in Kagawa were monomorphic. Especially,
two haplotypes endemic to Kagawa were randomly distributed, irrespective of drainages. Rhodeus o. kurumeus in Fukuoka inhabits small rivers and creeks (open water systems), while that in Kagawa and Osaka lives in small ponds (closed
water systems). Taking the information of morphology, mtDNA, and habitat into consideration, the low genetic diversity in
Kagawa and Osaka populations of R. o. kurumeus is thought to be mainly the result of the isolation of their habitat.
Received: January 14, 2001 / Revised: June 14, 2001 / Accepted: July 1, 2001 |
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Keywords: | D-loop Genetic diversity ND1 RFLP Rhodeus |
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