GM1 ELISA method for demonstration of Escherichia coli heat-stable enterotoxin |
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| Authors: | Ann-Mari Svennerholm Marianne Lindblad |
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| Institution: | Department of Medical Microbiology, University of Göteborg, Guldhedsgatan 10, S-413 46 Göteborg, Sweden |
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| Abstract: | Abstract A GM1-ELISA for detection of the methanol soluble, heat-stable enterotoxin (STa) produced by many enterotoxinogenic E. coli strains has been developed. This ST-GM1-ELISA, which is based on inhibition of binding of anti-ST antibody to GM1-bound ST-cholera B subunit conjugates, is relatively simple and possible to perform with stable reagents and without any complicated equipment. By this method STa could be detected in culture filtrates of human E. coli isolates with 100% sensitivity and specificity. The sensitivity of the method for purified ST is considerably higher than that of the conventional infant mouse test and comparable to that of recently described radioimmunoassays for ST. |
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| Keywords: | STa ST detection method ST immunoassay |
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