Binding of flunitrazepam to differentiating neurons cultured in a chemically defined,hormone-supplemented medium |
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| Authors: | Giovanni Savettieri Rosa Guarneri Giuseppe Salemi Vincenzo La Bella Donatella Ferraro Salvatore Scondotto Federico Piccoli |
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| Affiliation: | (1) Istituto di Neuropsichiatria, Università degli Studi di Palermo, Via G. La Loggia, 1-90129 Palermo, (Italy);(2) Istituto di Oftalmologia, Università degli Studi di Palermo, Via G. La Loggia, 1-90129 Palermo, (Italy);(3) Istituto di Microbiologia, Università degli Studi di Palermo, Via G. La Loggia, 1-90129 Palermo, (Italy) |
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| Abstract: | [3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. |
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| Keywords: | Flunitrazepam neuronal culture GABA |
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