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Isolation and properties of the natural and the recombinant sialidase fromClostridium septicum NC 0054714
Authors:Kirsten I Zenz  Peter Roggentin  Roland Schauer
Institution:(1) Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstr, 40, D-2300 Kiel, Germany
Abstract:The natural sialidase ofClostridium septicum was purified and characterized in parallel with the recombinant enzyme expressed byEscherichia coli. The two enzymes exhibit almost identical properties. The maximum hydrolytic activity was measured at 37 °C in 60mm sodium acetate buffer, pH 5.3. Glycoproteins like fetuin and saponified bovine submandibular gland mucin, most of them having agr(2-6) linked sialic acids, are preferred substrates, while sialic acids from gangliosides, sialyllactoses, or the agr(2-8) linked sialic acid polymer (colominic acid) are hydrolysed at lower rates. agr(2-3) Linkages are more rapidly hydrolysed than agr(2-6) bonds of sialyllactoses. The cleavage rate is markedly reduced by O-acetylation of the sialic acid moiety. These properties are similar to those of other secreted clostridial sialidases. The enzyme exists in mono-, di- and trimeric forms, the monomer exhibiting a molecular mass of 125 kDa, which is close to the protein mass of 111 kDa deduced from the nucleotide sequence of the cloned gene.Abbreviations BSM bovine submandibular gland mucine - CMM cooked meat medium - EDTA ethylenediaminetetraacetic acid - FPLC fast performance liquid chromatography - LB Luria-Bertani - MU-Neu5Ac 4-methylumbelliferyl-agr-d-N-acetylneuraminic acid - Neu5Ac N-acetylneuraminic acid - Neu5Ac2en 2-deoxy-2,3-didehydro-N-acetylneuraminic acid - Neu4,5Ac2 N-acetyl-4-O-acetylneuraminic acid - pI isoelectric point - SDS sodium dodecyl sulfate
Keywords:sialidase (neuraminidase)  cloned and natural enzyme  isolation  comparison of properties  Clostridium septicum
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