| 捷安肽素高产突变株96孔板筛选方法的建立 |
| |
| 引用本文： | 陈芬,张晓勇,钟娟,周金燕,李志东,杨杰,谭红.捷安肽素高产突变株96孔板筛选方法的建立[J].生物技术通报,2010(3). |
| |
| 作者姓名： | 陈芬 张晓勇 钟娟 周金燕 李志东 杨杰 谭红 |
| |
| 作者单位： | 1. 中国科学院成都生物研究所应用与环境微生物研究中心,成都,610041;中国科学院研究生院生命科学院,北京,100049
2. 中国科学院成都生物研究所应用与环境微生物研究中心,成都,610041 |
| |
| 基金项目： | 中国科学院知识创新工程重要方向项目 |
| |
| 摘 要： | 建立了一种快速灵敏地筛选出捷安肽素(JAA)高产突变株的方法。主要利用96多孔板固体培养菌体,用50%乙醇为最佳浸提液浸提4h,滤纸片法检测JAA生物活性的点样量为100μL,筛选出5株高产突变株,经摇瓶复筛选出2株高产突变株,经一剂量法检测,其抑菌圈直径较出发菌株提高了14%左右。
|
| 关 键 词： | 捷安肽素 96孔板 浸提 滤纸片法 初筛方法 |
Establishnent of a 96-well Microplate Screening Method for High Yield Strains of Jiean-peptide |
| |
| Chen Fen,Zhang Xiaoyong,Zhong Juan,Zhou Jinyan,Li Zhidong,Yang Jie,Tan Hong.Establishnent of a 96-well Microplate Screening Method for High Yield Strains of Jiean-peptide[J].Biotechnology Bulletin,2010(3). |
| |
| Authors: | Chen Fen Zhang Xiaoyong Zhong Juan Zhou Jinyan Li Zhidong Yang Jie Tan Hong |
| |
| Institution: | Chen Fen1,2 Zhang Xiaoyong1 Zhong Juan1 Zhou Jinyan1 Li Zhidong1 Yang Jie1 Tan Hong1(1Center for Applied , Environmental Microbiology,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,2College of Life Science,Graduate University of Chinese Academy of Sciences,Beijing 100049) |
| |
| Abstract: | A more rapid and sensitive screening method was constructed in this article in order to screen out the high-yielding mutants of Jiean-peptide(JAA).The method included solid culture strains in 96-well microplate,extraction active ingredients of JAA with 50% ethanol for four hours,the filter paper method to detect biological activity of JAA.100 μL was the most appropriate testing dosage for the filter paper method.Five high-yield mutants were obtained by the screening method.Two of them were chosen,through shake-flask fermentation,which ensured they were high-producing strains.Diameters of antiblastic circles of them were increased by about 14% compared with the starting strain through one dosage technique. |
| |
| Keywords: | Jiean-peptide 96-well Microplate Extraction Filter paper method Prescreening method |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
