Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle |
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Authors: | Luc Grobet Dominique Poncelet Luis José Royo Benoit Brouwers Dimitri Pirottin Charles Michaux François Ménissier Marta Zanotti Susana Dunner Michel Georges |
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Institution: | (1) Department of Genetics, Faculty of Veterinary Medicine, University of Liège (B43), 20 Bd de Colonster, 4000-Liège, Belgium, BE;(2) Laboratorio de Genetica, Dpto. de Produccion Animal, Universidad Complutense, 28040 Madrid, Spain, ES;(3) Station de Génétique Quantitative et Appliquée. I.N.R.A., 78352 Jouy-en-Josas, France, FR;(4) Istituto di Zootecnia, facolta di Medicina Veterinaria, Universita degli Studi di Milano, 20133 Milano, Italy, IT |
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Abstract: | We have determined the entire myostatin coding sequence for 32 double-muscled cattle sampled from ten European cattle breeds.
Seven DNA sequence polymorphisms were identified, of which five would be predicted to disrupt the function of the protein,
one is a conservative amino acid substitution, and one a silent DNA sequence variant. Four additional DNA sequence polymorphisms
were identified in myostatin intronic sequences. In all but two breeds, all double-muscled animals were either homozygous
or compound heterozygotes for one of the five loss-of-function mutations. The absence of obvious loss-of-function mutations
in the coding sequence of the two remaining breeds points either towards additional mutations in unexplored segments of the
gene, or towards locus heterogeneity of double-muscling.
Received: 20 September 1997 / Accepted: 11 October 1997 |
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