Poly-3-hydroxybutyrate production byAzotobacter chroococcum |
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Authors: | J Parshad S Suneja K Kukreja K Lakshminarayana |
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Institution: | (1) Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, 125 004 Hisar, India |
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Abstract: | Thirty-seven soil isolates and mutants ofAzotobacter chroococcum tested for poly-3-hydroxybutyrate (PHB) production using Sudan black B staining method were found to be positive. One mutant
showed a higher number of PHB-producing cells and maximum number of granules per cell. Using 2% glucose and 15 mmol/L ammonium
acetate, PHB production was found to be maximum at 36 and 48 h of growth under submerged cultivation and under stationary
cultivation, respectively. PHB production was found to be higher on sucrose and commercial sugar (as carbon sources) as compared
to glucose and mannitol. As commercial sugar is cheaper than sucrose it was selected as carbon source for PHB production,
that being found to be maximum at 1% concentration. Inorganic nitrogen sources seemed to have no stimulatory effect on the
production of PHB. However, ammonium acetate (15 mmol/L) was found to be best for PHB production. Peptone (0.2 %) gave a better
yield of PHB under both growth conditions. Using all optimized conditions, PHB production was studied in ten selected strains.
Two of them were found to be best PHB producers under both growth conditions, one producing 621 and 740 μg/g dry mass under
submerged cultivation and under stationary cultivation, respectively, while the second one produced 589 and 733 μg/g. |
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