Morphometric and enzyme histochemical behaviour of the kidney of young rats before and after unilateral nephrectomy |
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Authors: | Dr Jerzy Kazimierczak Dr Pierre Chavaz Dr Radivoj Krstić Dr Otto Bucher |
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Institution: | (1) Institute of Histology and Embryology of the University of Lausanne, Rue du Bugnon 9, CH-1011 Lausanne, Switzerland |
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Abstract: | Summary Four week old male Wistar rats were unilaterally nephrectomized, and one, two, four, fourteen, and twenty-eight days after
the operation the remaining kidneys were removed for morphological examination—including measurement of diameters of the tubules—and
for histochemical investigation of enzymatic activities in the nephrons. The following enzymes were examined: succinate DH,
lactate DH, α-glycerophosphate DH, isocitrate DH, glucose-6-phosphate DH, cytochrome oxidase, adenosine triphosphatase, alkaline
and acid phosphatases.
In comparison with kidneys of control animals of the same age and sex, the hypertrophy of the remaining kidney was found to
be, even in these young rats, entirely due to enlargement of preexisting nephrons. The measurements have indicated that the
hypertrophy was most distinct between the second and fourth day after uninephrectomy. Four weeks after the operation the diameters
of proximal and distal tubules were about 21% and 18% respectively bigger in the uninephrectomized animals than in the corresponding
controls.
This increase in size was accompanied by some changes in intensity of enzymatic staining reactions; after an intial diminishment,
a graduate increase in activity of almost all the observed tubular enzymes was found; this seems to be the expression of the
functional compensation by the remaining kidney.
Paper dedicated to Professor Dr. Drs. h. c. W. Bargmann (Kiel) in honour of the 70th anniversary of his birthday. |
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