| Affiliation: | (1) Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 305-333 Daejon, Korea;(2) Division of Genome Resources and Reservation, National Genome Research Institute, National Institute of Health, 122-701 Seoul, Korea;(3) Division of Life Science, Daejeon University, 300-716 Daejon, Korea;(4) Dongbu Research Council, Korea Native Plants Institutes, Daeduck Science Town, 305-333 Daejon, Korea |
| Abstract: | We sequenced the nuclear ribosomal internal transcribed spacer (ITS) regions to determined the phylogenetic relationships of the severalGoodyera species in Korea and to measure the extent of their differentiation region. ITS 1 was 238 to 239 bp long while ITS 2 was 258 to 259 bp. The 5.8S coding region was 156 bp long. Sequence divergences among species, calculated by Kimura’s two- parameter method, ranged from 0.0 to 5.4%. The most parsimonious tree, with a consistency index of 0.935 and a retention index of 0.937, was produced with 337 steps. Our ITS sequence results demonstrate the monophyly of KoreanGoodyera and support previous morphological, geographical, and RAPD data analyses. |
