| PCR-SSCP技术在微生物分类鉴定中的应用 |
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| 引用本文： | 方彩媛,张建丽,逄焕成,张雅博,樊金萍,王杰伟.PCR-SSCP技术在微生物分类鉴定中的应用[J].生物学杂志,2011,28(1):66-69. |
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| 作者姓名： | 方彩媛 张建丽 逄焕成 张雅博 樊金萍 王杰伟 |
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| 作者单位： | 1. 北京理工大学生命学院,北京,100081
2. 中国农业科学院农业资源与农业区划研究所,北京,100081 |
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| 基金项目： | 国家自然科学基金资助项目,公益性行业(农业)科研专项经费资助项目,北京理工大学基础研究基金资助项目 |
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| 摘 要： | PCR-SSCP是利用DNA单链构象具有多态性进行基因检测的一种分析技术,该技术敏感性高、操作简便,广泛应用于多种基因突变的检测和基因多态性分析,近年来,PCR-SSCP技术被大量应用于微生物学的研究中。综述了该技术的基本原理并主要对其在微生物学分类鉴定中的应用作了总结。
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| 关 键 词： | PCR-SSCP 微生物 分类鉴定 |
Application of PCR-SSCP in microbe's classification and determination |
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| FANG Cai-yuan,ZHANG Jian-li,PANG Huan-cheng,ZHANG Ya-bo,FAN Jin-ping,WANG Jie-wei.Application of PCR-SSCP in microbe's classification and determination[J].Journal of Biology,2011,28(1):66-69. |
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| Authors: | FANG Cai-yuan ZHANG Jian-li PANG Huan-cheng ZHANG Ya-bo FAN Jin-ping WANG Jie-wei |
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| Institution: | FANG Cai-yuan1,ZHANG Jian-li1,PANG Huan-cheng2,ZHANG Ya-bo1,FAN Jin-ping1,WANG Jie-wei1(1.School of Life Science and Technology,Beijing Institute of Technology,Beijing 100081,2.Institute of Agri-resources and Regional Planning,CAAS,China) |
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| Abstract: | PCR-SSCP is one of analytic technology to examine genome based on the DNA single strand conformation polymorphism.It is widely used in variety of gene mutations and genetic polymorphism analysis.Because it is sensitive and easy to operate,PCR-SSCP technology is extensively used in the study of microbiology.Its basic principle and application in microbial classification and determination were summarized in this paper. |
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| Keywords: | PCR-SSCP |
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