化州柚查尔酮合成酶基因克隆与序列分析

利用CTAB-LiCl法提取高质量的化州柚总RNA,采用RT-PCR技术克隆查尔酮合成酶基因,获得广东道地药材化橘红资源化州柚的查尔酮合成酶基因。该基因编码区全长1176bp,编码391个氨基酸残基,与同样来源于柑橘属的查尔酮合成酶基因同源性高达98%。CTAB-LiCl法能提取高质量的化州柚总RNA,可以用于后续基因克隆和分析;克隆获得的查尔酮合成酶具有编码区,与同属植物相同基因具有高度序列同源性。

Cloning and sequence analyzing of chalcone synthase gene in Citrus grandis'tomenxosa'

To clone chalcone synthase（CHS） gene from the Citrus grandis tomentosa,which was the genuine medicinal materials from Guangdong province,CTAB-LiCl treatment was used to extract RNA from Citrus grandis tomentosa′ and RT-PCR was used to clone the CHS gene at the same time.The CHS gene included the open reading frame（ORF）,and it was consisted of 1173bp nucleic acid,encoded 391 amino acid residues.Compared with other CHS genes from citrus plant,it was found that they shared a sequence homology more than 98% at the nucleic acid level.Result showed that CTAB-LiCl treatment could extract high quality RNA from Citrus grandis tomentosa′.The cloned CHS gene from Citrus grandis tomentosa′ had the open reading frame and shared a high sequence homology with other Citrus plant.