Improved cell growth in tobacco suspension cultures expressing Vitreoscilla hemoglobin |
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Authors: | Farrés Judith Kallio Pauli T |
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Affiliation: | Institute of Biotechnology, ETH-Zürich, CH-8093, Switzerland. |
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Abstract: | Expression of the gene encoding bacterial hemoglobin (VHb) from Vitreoscilla has been previously used to improve recombinant cell growth and enhance product formation under microaerobic conditions, a common phenomenon in large-scale cultivations of bacteria. This technology has now been applied to tobacco suspension cultures. Tobacco suspension cultures have been generated from VHb-expressing tobacco plants. Cell cultures were capable of producing an active hemoglobin. When grown in shake flasks, the cells did not show any lag-phase and exhibited improved cell growth, compared to controls carrying the parental plasmid. |
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