Induction of preneoplastic alterations by X rays and neutrons in exposed rat tracheas and isolated tracheal epithelial cells

The relative potential of high- and low-LET radiation to induce preneoplastic alterations in rat tracheal epithelial cells was evaluated using a combined in vivo-cell culture model. The capacity of X rays and high- and low-dose-rate neutrons to induce preneoplastic changes in isolated rat tracheal epithelial cells and in the intact tissue was compared. The presence of altered populations was determined in culture in terms of the frequency of tracheal epithelial cell populations which exhibit enhanced growth capacity in culture and in terms of the induction of persistent morphological alterations in exposed transplanted tracheas. Prior to assaying for altered cells, tracheal epithelial cells were irradiated as part of the intact tissue or as single cells. Low- and high-LET radiation induced similar maximum frequencies of altered cells when cultures were exposed as single cells, although high-LET radiation was more radiobiologically effective (RBE = 20) than low-LET radiation. The most marked difference between high- and low-LET radiation was observed after irradiation of the intact tissue. Damage induced by low-LET radiation, giving rise to altered populations, was modified in the intact tissue, whereas similar damage induced by high-LET radiation was apparently not.