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Transbilayer distribution of phospholipid synthesizing enzymes in the sarcoplasmic reticulum membrane
Authors:S Piku?a  G Szymańska  M G Sarza?a
Affiliation:1. Fujian Key Laboratory of Sensing and Computing for Smart Cities, School of Informatics, Xiamen University, Xiamen, China;2. School of Geospatial Engineering and Science, Sun Yat-sen University, Zhuhai, China;3. School of Big data and Computer Science, Guizhou Normal University, Guiyang, China;4. East China Normal University, Key Laboratory of Spatial-Temporal Big Data Analysis and Application of Natural Resources in Megacities (Ministry of Natural Resources), Shanghai, China;5. Department of Geography and Environmental Management and Department of Systems Design Engineering, University of Waterloo, Waterloo, Canada;1. MNR Key Laboratory for Geo-Environmental Monitoring of Great Bay Area & Guangdong Key Laboratory of Urban Informatics & Shenzhen Key Laboratory of Spatial Smart Sensing and Services, Shenzhen University, Shenzhen 518060, China;2. School of Architecture and Urban Planning, Shenzhen University, Shenzhen 518060, China;3. School of Artificial Intelligence, Shenzhen Polytechnic, Shenzhen 518055, China;4. State Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;5. School of Geographical Sciences, Hebei Normal University, Shijiazhuang 050024, China;6. School of Surveying and Geo-Informatics, Shandong Jianzhu University, Jinan 250101, China;1. Chemical and Biochemical Processing Division, ICAR – Central Institute for Research on Cotton Technology, Mumbai 400019, India;2. Department of Biology, East Carolina University, Greenville 27858, USA;3. Instrumental and Food Analysis Laboratory, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamilnadu 632014, India;4. Agro Produce Processing Division, ICAR—Central Institute of Agricultural Engineering, Bhopal 462038, India;5. ICAR – Indian Institute of Wheat and Barley Research, Karnal 132001, India;6. Department of Pharmacy, Bhagwant University, Ajmer, Rajasthan 305004, India;7. Department of Food Science and Technology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni 173230, India;8. Centro Tecnológico de la Carne de Galicia, rúa Galicia n 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain;9. Nammazhvar Organic Farming Research Centre, Tamil Nadu Agricultural University, 641003 Coimbatore, India;10. Bharati Vidyapeeth (Deemed to be University), Yashwantrao Mohite Institute of Management, Karad, India;11. Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia;12. Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt;13. Department of Medical Pharmacology, College of Medicine, Assiut University, Assiut 7111, Egypt;14. Department of Pharmacology, College of Pharmacy, Najran University, Najran, Saudi Arabia;15. Department of Animal Husbandry, Government of Kerala, Kerala 679335, India;p. Department of Life Sciences, Presidency University, 86/1 College Street, Kolkata, West Bengal, India;q. Department of Rural Development and Agricultural Extension, College of Agriculture, Wolaita Sodo University, Wolaita Sodo, Ethiopia;r. Dr. S. S. Bhatnagar University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh 160014, India;s. Division of Physiology, Biochemistry and Post-Harvest Technology, ICAR—Central Plantation Crops Research Institute (CPCRI), Kasaragod 671124, India;t. Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, India;u. Division of Horticulture, RCSM College of Agriculture, Kolhapur 416004, India;v. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland;1. Department of Soil, Plant and Food Sciences, University of Bari A. Moro, Via Amendola 165/A, 70126 Bari, Italy;2. Water Research Institute, IRSA-CNR, Viale F. De Blasio 5, Bari 70132, Italy;3. Computing Sciences, Tampere University, Korkeakoulunkatu 7, 33720 Tampere, Finland;1. Dept. of Telecommunications and Information Processing, Ghent University, St.-Pietersnieuwstraat 41, Ghent, B9000, Belgium;2. Dept. of Computer Science, San Francisco State University, San Francisco, CA, USA
Abstract:The topology of enzymes involved in phospholipid synthesis in the membrane of sarcoplasmic reticulum vesicles was investigated by means of limited proteolysis. The digestion of sarcoplasmic reticulum proteins with different proteases caused the considerable decrease of the activities of choline- and ethanolaminephosphotransferases without significant inactivation of glycerophosphate and lysophosphatidylcholine acyltransferases. On the basis of these results we concluded that the active sites of phosphotransferases are located on the cytoplasmic side, whereas the active sites of acyltransferase are present either on the extracytoplasmic side or burried into the sarcoplasmic reticulum membrane.
Keywords:
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