Adsorption of aspartase onto hydrophobic cloth for conversion of ammonium fumarate |
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| Authors: | Hiroshi Yamazaki Suniti Sharma Sushama Joshi |
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| Affiliation: | (1) Department of Biology and Institute of Biochemistry, Carleton University, K1S 5B6 Ottawa, Ontario, Canada |
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| Abstract: | Summary A simple aspartase assay was developed. Aspartase fromEscherichiacoli Crooks strain was adsorbed to  -naphthyl cotton cloth by hydrophobic interaction. The adsorbed enzyme did not desorb in 1 M ammonium fumarate. The adsorbed enzyme exhibited the same pH vs. activity curve as free enzyme and had a half life of approx. 40 weeks. A column packed with the adsorbed aspartase showed 100% conversion of 1 M ammonium fumarate at a space velocity of approx. 2. |
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